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KMID : 1024420220260030140
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Food Engineering Progress
2022 Volume.26 No. 3 p.140 ~ p.146
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A Study on the Isolation and Characterization of Aerobic Halophilic Microorganisms Isolated from the Soil Around the Port on Jeju Island
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Lee Yong-Jik
Ganbat Dariimaa
Jeong Ga-Eul
Shin Kee-Sun
Lee Sang-Jae
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Abstract
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To isolate aerobically and identify the diversity of halophilic bacteria in the soil around two ports, Daepopogu and Hwasun Port, on Jeju island, a total 46 halophilic bacteria strains were isolated and phylogenetically analysed. The isolated strains were divided into 3 phyla, 8 families, 16 genera and 23 species. The main taxa was the Bacilli class, which included 50.0% of the strains with 3 families, 10 genera and 15 species of Bacillaceae, Exiguobacterium_f and Planococcaceae. The second taxa was the Gammaproteobacteria class, which included 45.7% of the strains with 4 families, 5 genera and 7 species of Aeromonadaceae, Halomonadaceae, Marinobacteraceae and Vibrionaceae. The isolated strains were tested for hydrolytic enzymes, amylase, lipase and protease activity, and 31 strains showed activity of at least one enzyme. Furthermore, auxin activity was determined in 7 strains. This study showed that the isolated strains have possible applications in the food and agricultural industries and have importance as a genetic resource in Korea.
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KEYWORD
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Soil, Isolation, Halophiles, Characterization, Enzyme
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